一种用于蛋白质二硫键异构酶活性的快速灵敏检测方法。
A rapid and sensitive assay for protein disulphide isomerase activity.
作者信息
Myllylä R, Oikarinen J
出版信息
J Biochem Biophys Methods. 1983 Feb;7(2):115-21. doi: 10.1016/0165-022x(83)90045-3.
PMID:6187793
Abstract
An assay procedure for the determination of protein disulphide isomerase activity is presented. The method is based on the reactivation of randomly cross-linked RNAase, the extent of RNAase reactivation being determined from the degradation of radioactively labelled RNA. The method is rapid and sensitive and allows one to test a large number of samples simultaneously.
摘要
本文介绍了一种测定蛋白质二硫键异构酶活性的检测方法。该方法基于随机交联的核糖核酸酶的重新激活，核糖核酸酶的重新激活程度通过放射性标记RNA的降解来测定。该方法快速、灵敏，能够同时检测大量样品。
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