Cone R E, Rosenstein R W, Tite J, Ptak W, Gershon R K
J Immunol. 1983 May;130(5):2083-7.
Rabbits were immunized with TNP-specific Lyt-1+, 2- T cell-derived, antigen-binding proteins (PCI-F) released by T cells sensitized by skin painting with picrylchloride. The resulting antiserum (anti-PCI-F) bound to PCI-F and TNP-specific factors that suppressed delayed hypersensitivity (TSF) known to be comprised of PCI-F and Lyt-2+ -derived polypeptides released by cells sensitized by injection of trinitrobenzenesulfonic acid (TNBSF). Anti-PCI-F bound to T lymphocytes and 68,000 to 72,000 m.w. T cell surface proteins but not B cells on their surface proteins. Anti-PCI-F bound to both Lyt-1+ and Lyt-2+ T cells and surface proteins. A comparison of anti-PCI-F with anti-TSF indicates that anti-TSF contains specificity for Ly-2+ T cell-derived components of TSF and T cells not present in anti-PCI-F. The possibility of multiple isotypes of T cell receptors and antigen-binding molecules is discussed.
用三硝基苯（TNP）特异性的Lyt-1⁺、2⁻ T细胞衍生的抗原结合蛋白（PCI-F）对兔子进行免疫，该蛋白由用苦味酰氯皮肤致敏的T细胞释放。产生的抗血清（抗PCI-F）与PCI-F以及抑制迟发型超敏反应的TNP特异性因子（TSF）结合，已知TSF由PCI-F和通过注射三硝基苯磺酸致敏的细胞释放的Lyt-2⁺衍生多肽组成。抗PCI-F与T淋巴细胞以及分子量为68,000至72,000的T细胞表面蛋白结合，但不与B细胞表面蛋白结合。抗PCI-F与Lyt-1⁺和Lyt-2⁺ T细胞及表面蛋白均结合。抗PCI-F与抗TSF的比较表明，抗TSF对TSF中Lyt-2⁺ T细胞衍生成分以及抗PCI-F中不存在的T细胞具有特异性。文中讨论了T细胞受体和抗原结合分子存在多种同种型的可能性。
