Ehlin-Henriksson B, Clark E A, Jønsson V, Klein G
J Immunol. 1983 May;130(5):2448-52.
In the Jijoye-P3HR-1 family of Burkitt lymphoma sublines, the expression of the B lymphoblast-1 antigen, BB-1, identified by the monoclonal antibody described by Yokochi and colleagues, was found to be strictly related to the expression of the EBV receptor/C3 receptor (EBVR/C3R) complex. It was absent on the receptor-negative P3HR-1 line, present in the original receptor-positive Jijoye line, and reappeared in nonvirus producer sublines derived from P3HR-1 itself. We suggest the BB-1 antigen is related to the EBVR/C3R complex in the Jijoye family, either at the level of genetic or epigenetic determination or at the level of steric interaction on the cell membrane. In all probability, however, the BB-1 antigen is not identical to the receptor itself. It is also clear that a similar relationship does not necessarily apply to other cell lines. In the course of the studies, it was accidentally discovered that propagation of the P3HR-1 cells on newborn instead of fetal calf serum induces the concomitant expression of EBV receptors, C3 receptors, and the BB-1 antigen. The mechanism of this induction is obscure; it does not appear to be related to any significant change in the frequency of virus-producing cells.
在伯基特淋巴瘤亚系的Jijoye - P3HR - 1家族中，由横地及其同事描述的单克隆抗体鉴定出的B淋巴母细胞 - 1抗原（BB - 1）的表达，被发现与EB病毒受体/C3受体（EBVR/C3R）复合物的表达密切相关。在受体阴性的P3HR - 1细胞系上不存在该抗原，在原始的受体阳性Jijoye细胞系中存在，并且在源自P3HR - 1自身的无病毒产生的亚系中再次出现。我们认为，在Jijoye家族中，BB - 1抗原在遗传或表观遗传决定水平，或在细胞膜上的空间相互作用水平上，与EBVR/C3R复合物相关。然而，很可能BB - 1抗原与受体本身并不相同。同样清楚的是，类似的关系不一定适用于其他细胞系。在研究过程中，偶然发现P3HR - 1细胞在新生牛血清而非胎牛血清上培养会诱导EB病毒受体、C3受体和BB - 1抗原的同时表达。这种诱导的机制尚不清楚；它似乎与产生病毒的细胞频率的任何显著变化无关。
