Shivanandappa T, Krishnakumari M K
Acta Pharmacol Toxicol (Copenh). 1983 Jan;52(1):12-7. doi: 10.1111/j.1600-0773.1983.tb01069.x.
Weanling male albino rats were fed 100, 750 and 1500 p.p.m. of technical HCH for 90 days. There was marked testicular atrophy with reduced tubule size and spermatogenetic arrest at 1500 p.p.m. Histochemically, there was accumulation of cholesterol-positive lipids in the Sertoli cells and the Leydig cells of the atrophied testis and biochemical estimation revealed significant increase of total lipid and cholesterol contents. Activities of delta 5 3 beta hydroxysteroid dehydrogenase (delta 5 3 beta HSDH), 17 beta hydroxysteroid dehydrogenase (17 beta HSDH) and glucose-6-phosphate dehydrogenase (G-6-PDH) in the interstitial cells were markedly decreased suggesting steroidogenic inhibition in the Leydig cells of the atrophied testis due to HCH feeding.
将断乳雄性白化大鼠分别喂食含100、750和1500 ppm工业级六氯环己烷的饲料，持续90天。在1500 ppm组出现明显的睾丸萎缩，曲细精管尺寸减小，精子发生停滞。组织化学分析显示，萎缩睾丸的支持细胞和间质细胞中胆固醇阳性脂质积累，生化检测表明总脂质和胆固醇含量显著增加。间质细胞中δ5 3β羟类固醇脱氢酶（δ5 3βHSDH）、17β羟类固醇脱氢酶（17βHSDH）和葡萄糖-6-磷酸脱氢酶（G-6-PDH）的活性明显降低，提示喂食六氯环己烷导致萎缩睾丸的间质细胞类固醇生成受到抑制。
