Kamekura M, Onishi H
J Bacteriol. 1978 Jan;133(1):59-65. doi: 10.1128/jb.133.1.59-65.1978.
The halophilic nuclease of Micrococcus varians ATCC 21971 hydrolyzed thymidine 5'-monophospho-p-nitrophenyl ester at a rate that increased with the NaCl concentration up to saturation. The nuclease attacked RNA and DNA exonucleolytically and processively, producing 5'-mononucleotides. The molecular weight of the enzyme as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was 99,000, approximately the same as that previously determined for the native enzyme. Examination of amino acid composition showed that acidic amino acids were in high excess over basic amino acids.
变异微球菌ATCC 21971的嗜盐核酸酶水解胸苷5'-单磷酸对硝基苯酯的速率随NaCl浓度增加直至饱和而加快。该核酸酶以外切核酸酶的方式连续作用于RNA和DNA，产生5'-单核苷酸。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，该酶的分子量为99,000，与先前测定的天然酶分子量大致相同。氨基酸组成分析表明，酸性氨基酸大大超过碱性氨基酸。
