嗜盐微球菌嗜盐亚种嗜盐核酸酶H在调味剂5'-鸟苷酸商业生产中的应用
Application of halophilic nuclease H of Micrococcus varians subsp. halophilus to commercial production of flavoring agent 5'-GMP.
作者信息
Kamekura M, Hamakawa T, Onishi H
出版信息
Appl Environ Microbiol. 1982 Oct;44(4):994-5. doi: 10.1128/aem.44.4.994-995.1982.
Abstract
RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, and the yield was 805 mg from 5 g of RNA.
摘要
嗜盐微球菌亚种嗜盐变种肉汤培养上清液中的嗜盐核酸酶H在60℃下将RNA降解24小时，该上清液含有12%的氯化钠。由于污染的5'-核苷酸酶在这些条件下几乎没有活性，因此可以从反应混合物中回收生成的5'-鸟苷酸，5克RNA的产量为805毫克。
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本文引用的文献
1
Halophilic nuclease from a moderately halophilic Micrococcus varians.来自中度嗜盐变异微球菌的嗜盐核酸酶。
J Bacteriol. 1974 Aug;119(2):339-44. doi: 10.1128/jb.119.2.339-344.1974.
2
Properties of the halophilic nuclease of a moderate halophile, Micrococcus varians subsp. halophilus.嗜盐微球菌嗜盐亚种嗜盐核酸酶的特性
J Bacteriol. 1978 Jan;133(1):59-65. doi: 10.1128/jb.133.1.59-65.1978.
3
A medium for commercial production of the halophilic Micrococcus nuclease.用于嗜盐微球菌核酸酶商业生产的培养基。
Can J Microbiol. 1979 Sep;25(9):1113-6. doi: 10.1139/m79-172.
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