Application of halophilic nuclease H of Micrococcus varians subsp. halophilus to commercial production of flavoring agent 5'-GMP.
作者信息
Kamekura M, Hamakawa T, Onishi H
出版信息
Appl Environ Microbiol. 1982 Oct;44(4):994-5. doi: 10.1128/aem.44.4.994-995.1982.
Abstract
RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, and the yield was 805 mg from 5 g of RNA.
摘要
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本文引用的文献
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2
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3
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