Kamekura M, Hamakawa T, Onishi H
Appl Environ Microbiol. 1982 Oct;44(4):994-5. doi: 10.1128/aem.44.4.994-995.1982.
RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, and the yield was 805 mg from 5 g of RNA.
嗜盐微球菌亚种嗜盐变种肉汤培养上清液中的嗜盐核酸酶H在60℃下将RNA降解24小时，该上清液含有12%的氯化钠。由于污染的5'-核苷酸酶在这些条件下几乎没有活性，因此可以从反应混合物中回收生成的5'-鸟苷酸，5克RNA的产量为805毫克。
