Dowling L M, Parry D A, Sparrow L G
Biosci Rep. 1983 Jan;3(1):73-8. doi: 10.1007/BF01121573.
Although it has been assumed that the microfibrils in hard -keratin are members of the class of structures known as intermediate filaments (IF), no firm chemical evidence relating the low-sulfur proteins in hard -keratin to other IF proteins has yet been published. We now present primary sequence data for two components from wool keratin which show striking similarities with two IF proteins, desmin and vimentin. The sequences show marked homology, a heptad repeat and a 9.5-residue periodicity in the linear disposition of the acidic and the basic residues. These data thus provide the first evidence that the low-sulfur proteins in hard -keratin and the other IF proteins do indeed have both a similar structure and a common evolutionary origin.
尽管人们一直认为硬角蛋白中的微原纤维是被称为中间丝（IF）的结构类别的成员，但尚未发表确凿的化学证据来证明硬角蛋白中的低硫蛋白与其他中间丝蛋白有关。我们现在展示了羊毛角蛋白中两种成分的一级序列数据，这些数据与两种中间丝蛋白——结蛋白和波形蛋白有显著相似性。这些序列显示出明显的同源性、七肽重复以及酸性和碱性残基线性排列中的9.5个残基周期性。因此，这些数据首次证明硬角蛋白中的低硫蛋白与其他中间丝蛋白确实具有相似的结构和共同的进化起源。
