人髓鞘碱性蛋白肽43-88的肾脏蛋白水解活性的物种比较。

Species comparison of renal proteolytic activity for human myelin basic protein peptide 43-88.

Whitaker J N, Heinemann M A

Comp Biochem Physiol B. 1983;74(3):445-8. doi: 10.1016/0305-0491(83)90208-0.

Abstract

A species comparison was conducted on the proteolytic activity in human, dog, rabbit, guinea-pig and rat kidney which can degrade human myelin basic protein peptide 43-88. 2. In rat kidney the degrading activity occurred over a pH range of 4-11.5 with the greatest activities at pH 5 and 9. The peptide degrading activity in human, dog, rabbit and guinea-pig kidney was considerably less than in the rat and occurred predominantly at pH 7 with lesser activity at pH 9. 3. The effects of inhibitors of proteolytic enzymes indicated that the peptide degrading activities at the same two pH's of dog, rabbit and guinea-pig were similar to one another but differed from that of human. 4. These results indicate that the activity for degrading a potential autoantigenic material is widespread in renal tissue among different species and that different enzymes are involved. More generally, these findings suggest that renal proteinases differ among commonly used laboratory animals and also differ from the human enzymes.

摘要

对人、狗、兔、豚鼠和大鼠肾脏中能够降解人髓鞘碱性蛋白肽43 - 88的蛋白水解活性进行了物种比较。2. 在大鼠肾脏中，降解活性发生在pH值4 - 11.5的范围内，在pH值5和9时活性最高。人、狗、兔和豚鼠肾脏中的肽降解活性明显低于大鼠，且主要发生在pH值7时，在pH值9时活性较低。3. 蛋白水解酶抑制剂的作用表明，狗、兔和豚鼠在相同两个pH值下的肽降解活性彼此相似，但与人类不同。4. 这些结果表明，在不同物种的肾组织中，降解潜在自身抗原性物质的活性普遍存在，且涉及不同的酶。更普遍地说，这些发现表明常用实验动物中的肾蛋白酶与人类酶不同，且不同实验动物之间的肾蛋白酶也存在差异。