血红蛋白Lepore波士顿型、血红蛋白A2和血红蛋白A的比较研究。
Comparative studies of Hb Lepore Boston, Hb A2, and Hb A.
作者信息
Adachi K, Asakura T, Gill F M, Schwartz E
出版信息
J Biol Chem. 1978 Jan 25;253(2):382-4.
Several functional tests were performed to compare Hb A, Hb A2, and Hb Lepore Boston, which has a delta-beta crossover in the region of residues 87 to 116. Oxygen equilibrium curves determined by an automatic apparatus in 0.1 M potassium phosphate buffer, pH 7.0, at 20 degrees showed that the p50 was 5.8 mm Hg for Hb Lepore Boston, in contrast to 8.1 and 10.3 mm Hg for Hb A2 and Hb A, respectively. The n values (Hill coefficinets) of Hb Lepore Boston and Hb A2 were slightly smaller than that of Hb A. The effect of 2,3-diphosphoglycerate and inositolhexophosphate on the p50 of Hb Lepore Boston and Hb A2 was less than that on the p50 of Hb A. The molecular stability to mechanical shaking of Hb Lepore Boston and Hb A2 showed that the oxy forms of Hb Lepore Boston and Hb A2 denatured at a rate 3 times faster than that of Hb A. MetHb Lepore Boston was more unstable than MetHb A2 to mechanical shaking. These results indicate that, although the molecular stability of Hb Lepore Boston is more similar to that of Hb A2 than that of Hb A, the oxygen-binding properties of Hb Lepore differ from both Hb A and Hb A2.
进行了多项功能测试，以比较Hb A、Hb A2和Hb Lepore Boston，后者在87至116位残基区域存在δ-β交叉。在20摄氏度下，由自动仪器在pH 7.0的0.1 M磷酸钾缓冲液中测定的氧平衡曲线显示，Hb Lepore Boston的p50为5.8 mmHg，相比之下，Hb A2和Hb A的p50分别为8.1和10.3 mmHg。Hb Lepore Boston和Hb A2的n值（希尔系数）略小于Hb A的n值。2,3-二磷酸甘油酸和肌醇六磷酸对Hb Lepore Boston和Hb A2的p50的影响小于对Hb A的p50的影响。Hb Lepore Boston和Hb A2对机械振荡的分子稳定性表明，Hb Lepore Boston和Hb A2的氧合形式变性速度比Hb A快3倍。高铁血红蛋白Hb Lepore Boston对机械振荡的稳定性低于高铁血红蛋白Hb A2。这些结果表明，尽管Hb Lepore Boston的分子稳定性与Hb A2比与Hb A更相似，但其氧结合特性与Hb A和Hb A2均不同。
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