Brushart T M, Mesulam M M
Neurosci Lett. 1980 Apr;17(1-2):1-6. doi: 10.1016/0304-3940(80)90051-8.
Transganglionic transport of horseradish peroxidase (HRP) was greatly improved by conjugating the enzyme with wheat germ agglutinin (WGA) as described by Gonatas et al. [7]. Intense dorsal horn projections from muscle groups and even discrete skin patches were labeled after peripheral injection of the HRP-WGA conjugate. These projections could not be consistently demonstrated when HRP was injected either alone or in combination with DMSO. Conjugation with WGA thus enhances the uptake and/or transport of HRP and constitutes a valuable advance in HRP technique.
如戈纳塔斯等人[7]所述，通过将辣根过氧化物酶（HRP）与麦胚凝集素（WGA）结合，大大改善了HRP的跨神经节运输。在外周注射HRP-WGA结合物后，肌肉群甚至离散的皮肤区域有强烈的背角投射被标记。当单独注射HRP或与二甲基亚砜联合注射时，这些投射无法持续显示。因此，与WGA结合可增强HRP的摄取和/或运输，这是HRP技术的一项有价值的进展。
