Litvak S, Sarih L, Fournier M, von der Haar F, Labouesse B, Araya A
Recent Results Cancer Res. 1983;84:184-90. doi: 10.1007/978-3-642-81947-6_13.
We have previously studied the topographical and functional implications of the recognition of primer tRNATrp by avian retrovirus reverse transcriptase. Here we have presented evidence that the enzyme is able to deacylate beef liver Trp-tRNATrp, provided that 35-S viral RNA is present in the incubation mixture. No effect of dNTPs on this activity was observed. The extensive modification of tRNATrp with acrylonitrile led to a marked loss of priming activity by tRNATrp if the annealing between primer and template was performed at 37 degrees C, while the annealing of cyanoethylated tRNA with the viral genome at 75 degrees C gave almost normal levels of cDNA synthesis. We have also studied the priming behaviour of tRNATrp, modified by incorporation of various analogs of adenosine. Only tRNATrp-2'dA was active in cDNA initiation; 3'dA, 3'NH2-3'dA, and primer tRNA with formycin in the 3' end showed low or nonexistent priming activity.
我们之前研究了禽逆转录病毒逆转录酶识别引物tRNATrp的拓扑学和功能意义。在此，我们提供了证据表明，只要在孵育混合物中存在35-S病毒RNA，该酶就能使牛肝Trp-tRNATrp脱酰基。未观察到dNTPs对该活性有影响。用丙烯腈对tRNATrp进行广泛修饰后，如果引物与模板之间的退火在37℃进行，tRNATrp的引发活性会显著丧失，而氰乙基化tRNA与病毒基因组在75℃退火时，cDNA合成水平几乎正常。我们还研究了通过掺入各种腺苷类似物修饰的tRNATrp的引发行为。只有tRNATrp-2'dA在cDNA起始中具有活性；3'dA、3'NH2-3'dA以及3'端带有间型霉素的引物tRNA显示出低引发活性或无引发活性。
