Schimmel R J, McCarthy L, McMahon K K
Am J Physiol. 1983 May;244(5):C362-8. doi: 10.1152/ajpcell.1983.244.5.C362.
Respiration was increased approximately 5-fold with 0.05 microM norepinephrine and to a maximum of 10-fold by 0.30 microM norepinephrine. Prazosin, an alpha-adrenergic blocking agent highly selective for alpha 1-type receptors, partially inhibited the response to norepinephrine (0.05 microM) by 20-25% at a concentration of 0.10-1 microM. In contrast, when the stimulus for respiration was provided by isoproterenol or 3-isobutyl-1-methylxanthine, prazosin was without effect up to a concentration of 10 microM. Yohimbine, an alpha-adrenergic blocking drug preferential for alpha 2-receptors, did not influence norepinephrine-stimulated oxygen uptake. Respiration was increased two- to fourfold by phenylephrine or methoxamine, agents preferential for alpha 1-adrenergic receptors but not at all by clonidine, an agent preferential for alpha 2-adrenergic receptors. The stimulatory effect of phenylephrine on oxygen uptake was fully blocked by prazosin but not propranolol. Removal of extracellular calcium with ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid prevented phenylephrine stimulation of respiration but was without effect when isoproterenol was the stimulus. These results support the participation of alpha 1-adrenergic receptors in control of respiration and are consistent with the possibility that changes in cell calcium are intimately involved in this response.
用0.05微摩尔去甲肾上腺素时呼吸增加约5倍，用0.30微摩尔去甲肾上腺素时呼吸最多增加10倍。哌唑嗪是一种对α1型受体具有高度选择性的α肾上腺素能阻断剂，在浓度为0.10 - 1微摩尔时，可部分抑制对去甲肾上腺素（0.05微摩尔）的反应达20 - 25%。相比之下，当由异丙肾上腺素或3 - 异丁基 - 1 - 甲基黄嘌呤提供呼吸刺激时，哌唑嗪在浓度高达10微摩尔时均无作用。育亨宾是一种对α2受体有选择性的α肾上腺素能阻断药，不影响去甲肾上腺素刺激的氧摄取。苯肾上腺素或甲氧明可使呼吸增加2至4倍，这两种药物对α1肾上腺素能受体有选择性，但对α2肾上腺素能受体有选择性的可乐定则完全无此作用。苯肾上腺素对氧摄取的刺激作用被哌唑嗪完全阻断，但不被普萘洛尔阻断。用乙二醇双（β - 氨基乙基醚） - N，N' - 四乙酸去除细胞外钙可阻止苯肾上腺素对呼吸的刺激，但当刺激物为异丙肾上腺素时则无此作用。这些结果支持α1肾上腺素能受体参与呼吸控制，并且与细胞钙变化密切参与该反应的可能性一致。
