Cunningham M
Anal Biochem. 1983 Feb 1;128(2):415-21. doi: 10.1016/0003-2697(83)90395-0.
Small aliquots of DNA-containing samples from gradient or column fractions are spotted onto agarose plates containing ethidium bromide, and the DNA is visualized qualitatively. After it is overlaid with a thin agarose cover, the DNA is denatured and transferred to nitrocellulose filters for hybridization. This method, called the "spot-blot assay," is particularly convenient when dealing with large numbers of samples.
从梯度或柱分级分离物中取少量含DNA的样品点样到含有溴化乙锭的琼脂糖平板上，对DNA进行定性观察。在其上覆盖一层薄的琼脂糖覆盖层后，使DNA变性并转移到硝酸纤维素滤膜上进行杂交。这种方法称为“斑点印迹分析”，在处理大量样品时特别方便。
