Chapel A, Bensussan A, Vilmer E, Dormont D
Laboratoire de Neuropathologie Expérimentale et Neurovirologie, Centre de Recherches du Service de Santé des Armées, Fontenay aux Roses, France.
J Virol. 1992 Jun;66(6):3966-70. doi: 10.1128/JVI.66.6.3966-3970.1992.
By using cloning methodology, 13 CD4+, CD8-, CD45RO+, and CD29+ clones, isolated from human immunodeficiency virus (HIV)-negative donors, have been characterized and tested regarding their susceptibility to two strains of HIV type 1 (HIV-1). Infected clones possess integrated provirus. Only six are able to replicate HIV-1, while seven may normally grow without cytopathic effect and without viral replication. These results argue that all CD4+ lymphocyte clones may be infectable but that a heterogeneity exists regarding their abilities to replicate HIV-1.
通过使用克隆方法，从人类免疫缺陷病毒（HIV）阴性供体中分离出13个CD4 +、CD8 -、CD45RO +和CD29 +克隆，并对它们对两株1型人类免疫缺陷病毒（HIV-1）的易感性进行了表征和测试。被感染的克隆具有整合的前病毒。只有六个能够复制HIV-1，而七个通常可以在无细胞病变效应且无病毒复制的情况下生长。这些结果表明，所有CD4 +淋巴细胞克隆都可能被感染，但它们复制HIV-1的能力存在异质性。
