Burt D S, Stanworth D R
Biochim Biophys Acta. 1983 Jun 2;762(3):458-65. doi: 10.1016/0167-4889(83)90012-5.
The role of cyclic AMP in the secretory mechanism of mast cells has been investigated by comparing the time course of changes in cellular levels of this cyclic nucleotide with the kinetics of secretion induced by basic peptides, antigen, anti-IgE and calcium ionophore. ACTH(1-24) peptide and a synthetic decapeptide representative of the sequence 497-506 within the C epsilon 4 domain of human IgE induced a transient rise in cyclic AMP which reached approx. 150% of the resting levels by 10 s. Peptide-induced secretion of histamine was also rapid, reaching a maximum after 5-10 s. Immunological triggering of mast cells with antigen and anti-IgE raised levels of cyclic AMP to 150% of resting levels within 15 s, accompanying secretion of histamine which reached a maximum after 30 s. A relatively slower release of histamine induced by the calcium ionophore A23187 was paralleled by a significant reduction in cyclic AMP to 50% of the resting levels after 300 s. These data suggest a relationship between the accumulation of cyclic AMP in mast cells and secretion of histamine mediated by the C epsilon 4 decapeptide and the ACTH(1-24) peptide as well as by IgE-dependent mechanisms. However, the simultaneous increase in cyclic AMP and secretion of histamine suggests that the two events may not be causally related.
通过比较这种环核苷酸的细胞水平变化的时间进程与碱性肽、抗原、抗IgE和钙离子载体诱导的分泌动力学，研究了环磷酸腺苷（cAMP）在肥大细胞分泌机制中的作用。促肾上腺皮质激素（1-24）肽和一种代表人类IgE Cε4结构域中497-506序列的合成十肽诱导cAMP短暂升高，在10秒时达到静止水平的约150%。肽诱导的组胺分泌也很快，在5-10秒后达到最大值。用抗原和抗IgE对肥大细胞进行免疫触发，可在15秒内使cAMP水平升高至静止水平的150%，同时组胺分泌在30秒后达到最大值。钙离子载体A23187诱导的组胺释放相对较慢，300秒后cAMP显著降低至静止水平的50%。这些数据表明，肥大细胞中cAMP的积累与Cε4十肽和促肾上腺皮质激素（1-24）肽以及IgE依赖性机制介导的组胺分泌之间存在关联。然而，cAMP和组胺分泌同时增加表明这两个事件可能没有因果关系。
