Chernicky C L, Barnes K L, Ferrario C M, Conomy J P
Brain Res Bull. 1983 Mar;10(3):345-51. doi: 10.1016/0361-9230(83)90102-8.
The distribution within the brainstem of cell bodies and efferent fibers projecting in the cervical vagus was studied with retrograde transport of horseradish peroxidase (HRP). Five to eight days after multiple microinjections of HRP into either the cervical vagosympathetic trunk or the nodose ganglion the brainstems and nodose ganglia were perfused and processed by the tetramethyl benzidine method. HRP-positive neurons were found in three brainstem regions: a dorsal cell column comprising the dorsal motor nucleus of the vagus (dmnX), a ventrolateral group in the region of nucleus ambiguus (nA), and scattered cells along a line between these columns. The density of labeled neurons was greatest within dmnX. Axons from cells of the ventrolateral column projected dorsomedially; just ventral to dmnX they turned laterally to exit the medulla in multiple rootlets. Within nA labelled neurons were distributed according to size, with larger cells more medial and smaller ones more lateral. Caudal to nA in nucleus retroambigualis and nucleus dorsalis medialis cell bodies appeared segregated into clusters.
采用辣根过氧化物酶（HRP）逆行运输法，研究了投射至颈迷走神经的细胞体和传出纤维在脑干内的分布情况。在颈迷走交感干或结状神经节多次微量注射HRP后5至8天，对脑干和结状神经节进行灌注，并采用四甲基联苯胺法进行处理。在三个脑干区域发现了HRP阳性神经元：一个背侧细胞柱，由迷走神经背运动核（dmnX）组成；在疑核（nA）区域的腹外侧组；以及沿这些柱之间的一条线分布的散在细胞。标记神经元的密度在dmnX内最大。腹外侧柱细胞的轴突向背内侧投射；在dmnX正腹侧，它们转向外侧，以多个小根离开延髓。在nA内，标记神经元根据大小分布，较大的细胞更靠内侧，较小的细胞更靠外侧。在疑核尾侧，在最后区核和内侧背核中，细胞体似乎聚集成簇。
