Kalia M, Sullivan J M
J Comp Neurol. 1982 Nov 1;211(3):248-65. doi: 10.1002/cne.902110304.
The sensory and motor connections of the cervical vagus nerves and of its inferior ganglion (nodose ganglion) have been traced in the medulla and upper cervical spinal cord of 16 male Wistar rats by using horseradish peroxidase (HRP) neurohistochemistry. The use of tetramethyl benzidine (TMB) as the substrate for HRP permitted the visualization of transganglionic and retrograde transport in sensory nerve terminals and perikarya, respectively. The vagus nerve in the rat enters the medulla in numerous fascicles with points of entry covering the entire lateral aspect of the medulla extending from level +4 to -6 mm rostrocaudal to the obex. Fascicles of vagal sensory fibers enter the dorsolateral aspect of the medulla and travel to the tractus solitarius (TS) which was labeled for over 8.8 mm in the medulla. The caudal extent of the TS receiving vagal projections was found in lamina V of the cervical spinal cord (C1 to C2). Sensory terminal fields could be visualized bilaterally in the nucleus of the tractus solitarius (nTS), area postrema (ap) and dorsal motor nucleus of the vagus nerve (dmnX). The ipsilateral projection to the nTS and the dmnX was heavier than that found on the contralateral side. The area postrema was intensely labeled on both sides. Motor fibers from HRP-labeled perikarya in the dmnX travel ventromedially in a distinct fascicle and subsequently subdivide into a number of small fiber bundles that traverse the medullary reticular formation in the form of a fine network of HRP-labeled fibers. As these fibers from the dmnX approach the ventrolateral aspect of the medulla they are joined by axons from the nucleus ambiguus (nA), nucleus retroambigualis (nRA) and the retrofacial nucleus (nRF). These latter fibers form hairpin loops in the middle of the reticular formation to accompany the axons from the dmnX exiting from the medulla in a ventrolateral location. HRP-labeled perikarya, in contrast to transganglionically transported HRP in sensory terminals in the nTS, were visualized on one side only, thus indicating that motor control via the vagus nerve is exerted only by motor neurons located ipsilaterally. Sensory information on the other hand, diverges to many nuclear subgroups located on both sides of the medulla.
利用辣根过氧化物酶（HRP）神经组织化学技术，在16只雄性Wistar大鼠的延髓和上颈段脊髓中追踪了颈迷走神经及其下神经节（结状神经节）的感觉和运动连接。使用四甲基联苯胺（TMB）作为HRP的底物，分别使感觉神经末梢和神经周体中的跨神经节运输和逆行运输可视化。大鼠的迷走神经以许多束的形式进入延髓，进入点覆盖延髓的整个外侧，从闩部尾端至头端+4至-6毫米处。迷走感觉纤维束进入延髓的背外侧，通向孤束（TS），孤束在延髓中标记长度超过8.8毫米。接受迷走神经投射的孤束的尾端范围见于颈段脊髓（C1至C2）的V层。感觉终末野可在孤束核（nTS）、最后区（ap）和迷走神经背运动核（dmnX）双侧可视化。向孤束核和迷走神经背运动核的同侧投射比在对侧发现的投射更密集。双侧最后区均被强烈标记。来自迷走神经背运动核中HRP标记神经周体的运动纤维在一个独特的束中向腹内侧走行，随后分成许多小纤维束，以HRP标记纤维的精细网络形式穿过延髓网状结构。当这些来自迷走神经背运动核的纤维接近延髓腹外侧时，它们与来自疑核（nA）、疑后核（nRA）和面神经后核（nRF）的轴突会合。后一组纤维在网状结构中部形成发夹环，伴随来自迷走神经背运动核的轴突在腹外侧位置离开延髓。与孤束核感觉末梢中经跨神经节运输的HRP不同，HRP标记的神经周体仅在一侧可视化，因此表明通过迷走神经的运动控制仅由同侧的运动神经元发挥作用。另一方面，感觉信息发散到位于延髓两侧的许多核亚群。
