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对未成熟虹鳟(Salmo gairdneri)垂体中促甲状腺激素细胞性质的组织生理和免疫细胞化学研究。

Histophysiological and immunocytochemical study on the nature of the thyrotrops in the pituitary of immature rainbow trout, Salmo gairdneri.

作者信息

van Putten L J, van Oordt P G, Terlou M, Peute J

出版信息

Cell Tissue Res. 1983;231(1):185-98. doi: 10.1007/BF00215785.

DOI:10.1007/BF00215785
PMID:6189619
Abstract

Alcian blue- and periodic acid-Schiff-positive "granular basophils" with electron-dense granules +/- 160 nm in diameter, and weakly developed irregular cisternae of the granular endoplasmic reticulum (GER) are present in the rostral pars distalis (RPD) and the proximal pars distalis (PPD) of immature rainbow trout. They all react with antisalmon alpha beta-gonadotropin. However, only granular basophils in the caudal RPD and the rostro-dorsal PPD, often bordering on and sometimes surrounded by neurophypophysial tissue, react with anti-human beta-TSH. These cells, considered as the source of thyrotropin, show degranulation and dilatation of the GER-cisternae in fish treated with potassium perchlorate. The thyroids of the goitrogen-treated animals had relatively numerous small follicles with a high epithelium. The remaining granular basophils are gonadotrops.

摘要

在未成熟虹鳟的吻端远侧部(RPD)和近侧部(PPD)中,存在阿尔辛蓝和过碘酸希夫染色阳性的“颗粒性嗜碱性细胞”,其具有直径约160纳米的电子致密颗粒,以及发育较弱的颗粒内质网(GER)不规则池。它们均与抗鲑鱼αβ促性腺激素发生反应。然而,只有尾侧RPD和吻背侧PPD中的颗粒性嗜碱性细胞,常与神经垂体组织相邻且有时被其包围,会与抗人β促甲状腺激素发生反应。这些细胞被认为是促甲状腺激素的来源,在用高氯酸钾处理的鱼中,它们表现出GER池的脱颗粒和扩张。甲状腺肿动物的甲状腺有相对较多的小滤泡,上皮较高。其余的颗粒性嗜碱性细胞是促性腺激素细胞。

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SEASONAL CHANGES IN THE CYTOLOGY OF THE ADENOHYPOPHYSIS, TESTES, AND OVARIES OF THE BLACK SURFPERCH, EMBIOTOCA JACKSONI, A VIVIPAROUS PERCOMORPH FISH.胎生鲈形目鱼类——杰克逊氏银汉鱼的腺垂体、睾丸和卵巢细胞结构的季节性变化
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The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中,将高pH值的柠檬酸铅用作电子不透明染色剂。
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Embedding in epoxy resins for ultrathin sectioning in electron microscopy.嵌入环氧树脂用于电子显微镜超薄切片。
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