van den Hurk R, Gielen J T, Terlou M
Cell Tissue Res. 1984;235(3):635-42. doi: 10.1007/BF00226962.
Effects of steroids on the accumulation of glycoprotein gonadotropin (GTH) in pituitaries of juvenile trout were investigated by means of scanning cytophotometry applied to immunocytochemical preparations, and with the use of a radioimmunoassay. Effects on other aspects of GTH-cell activity were analyzed by measuring the size of the gonadotrops and their nuclei. Progesterone added to aquarium water and methyltestosterone incorporated into the food showed a pronounced stimulatory effect on the accumulation of GTH. To a lesser extent, treatment with cortisol, cortisone, and desoxycorticosterone acetate administered to aquarium water, and 11 beta-hydroxy-androstenedione added to the food resulted in an increase of the hypophysial content of GTH. Steroids stimulating the accumulation of GTH in the pituitary also exhibited a positive effect on GTH-cell activity as indicated by an increase in the size of gonadotropic cells. Progesterone incorporated into the food did not influence the GTH-content and the GTH-cell activity. It is suggested that the route of administration of an exogenous steroid is essential for its effect on GTH cells in trout. Comparison of GTH values reveals an excellent correlation between the data from the radioimmunoassay and those from the corresponding densitometric measurements. No correlation was observed between values of morphometrically determined GTH-cell activity and the densitometric values reflecting hypophysial GTH content.
通过应用于免疫细胞化学制剂的扫描细胞光度法以及放射免疫测定法，研究了类固醇对幼年鳟鱼垂体中糖蛋白促性腺激素（GTH）积累的影响。通过测量促性腺细胞及其细胞核的大小，分析了对GTH细胞活性其他方面的影响。添加到水族箱水中的孕酮和添加到食物中的甲基睾酮对GTH的积累具有显著的刺激作用。在较小程度上，向水族箱水中施用皮质醇、可的松和醋酸脱氧皮质酮，以及向食物中添加11β-羟基雄烯二酮，导致垂体中GTH含量增加。刺激垂体中GTH积累的类固醇对GTH细胞活性也有积极影响，表现为促性腺细胞大小增加。添加到食物中的孕酮不影响GTH含量和GTH细胞活性。提示外源性类固醇的给药途径对其对鳟鱼GTH细胞的作用至关重要。GTH值的比较显示，放射免疫测定数据与相应光密度测量数据之间具有良好的相关性。形态学测定的GTH细胞活性值与反映垂体GTH含量的光密度值之间未观察到相关性。
