使用不同批次、不同存放时间的¹²⁵I对人生长激素进行放射性碘化。

Human growth hormone radioiodination using different batches of 125I of various ages.

作者信息

Bartolini P, Camillo M A

出版信息

Clin Chim Acta. 1983 Apr 25;129(3):353-8. doi: 10.1016/0009-8981(83)90039-6.

Abstract

The reproducibility and the influence of the batch and decay level of Na125I on the radioiodination of human growth hormone (hGH) were examined by a polyacrylamide gel electrophoresis (PAGE) technique. The between-day coefficient of variation (CV) exhibited a value of 11.9% for labelling yields and 14.3% for antibody specific binding. The within-day variation for different shipments and isotope decay-levels was similar to that for the control experiment, carried out under the same conditions, using Na125I from a single lot. The results indicate that the decay-level and batch do not significantly influence either the yields or the immunological properties of the labelled product. The suitability of this PAGE technique as a control test for radioiodinated proteins is established by comparison with gel chromatography on Sephadex G-100.

摘要

采用聚丙烯酰胺凝胶电泳（PAGE）技术研究了Na125I的批次和衰变水平对人生长激素（hGH）放射性碘化的重现性及影响。日间变异系数（CV）显示，标记产率的变异系数为11.9%，抗体特异性结合的变异系数为14.3%。不同批次货物和同位素衰变水平的日内变异与在相同条件下使用来自同一批次的Na125I进行的对照实验相似。结果表明，衰变水平和批次对标记产物的产率或免疫特性均无显著影响。通过与Sephadex G - 100凝胶色谱法比较，确定了该PAGE技术作为放射性碘化蛋白质对照试验的适用性。