Dabelsteen E, Fejerskov O, Norén O, Mackenzie I C
J Invest Dermatol. 1978 Jan;70(1):11-5. doi: 10.1111/1523-1747.ep12543358.
Fluorescein conjugates of concanavalin A (Con-A) and Ricinus communis fraction 120 (RCA120) were shown to bind to the cell surfaces of basal and spinous cell layers in oral buccal mucosa. Palatal epithelium showed distinct binding to basal and spinous cells; cell membranes in the granular layer occasionally bound Con-A and always RCA120. The ultrastructural localization of Con-A binding sites on exfoliated buccal cells was detected by the Con-A peroxidase staining method. The Con-A receptors were seen on the cell surface in association with the outer leaflet of the plasma membrane. The reaction products appeared as a homogeneous, electron-dense layer containing irregularly distributed globules.
伴刀豆球蛋白A（Con-A）和蓖麻凝集素120（RCA120）的荧光素缀合物已被证明可与口腔颊黏膜基底层和棘细胞层的细胞表面结合。腭上皮显示出与基底细胞和棘细胞有明显的结合；颗粒层中的细胞膜偶尔结合Con-A，且总是结合RCA120。通过Con-A过氧化物酶染色法检测了Con-A结合位点在脱落颊细胞上的超微结构定位。在细胞表面观察到Con-A受体与质膜的外小叶相关联。反应产物表现为一层均匀的、电子致密层，含有不规则分布的小球。
