Mizukawa Y, Takata K, Ookusa Y, Nagashima M, Hirano H
Department of Dermatology, Kyorin University School of Medicine, Tokyo, Japan.
Histochem J. 1994 Nov;26(11):863-9. doi: 10.1007/BF00162932.
The pattern of lectin binding in normal human labial mucosa was examined by light and electron microscopy using eight different lectins (ConA, LCA, WGA, UEA-1, RCA-1, SBA, DBA and PNA) and compared with the patterns in normal human skin and oesophageal mucosa. As seen by light microscopy, ConA, LCA, and WGA stained cell membranes in all layers of the mucosae. RCA-1 stained the plasma membrane of cells in the basal and middle layers, whereas cells in the superficial layers showed little positive staining. UEA-1, SBA, and PNA stained the cells in the middle layers weakly in some cases. No positive staining for DBA was seen. By electron microscopy, reaction product indicating ConA-binding sites was observed in the plasma membrane, cisternae of the endoplasmic reticulum, nuclear envelope and the Golgi apparatus. Binding of LCA, WGA, and RCA-1 was observed in the plasma membrane. These results show that the binding pattern of PNA, SBA, and RCA-1 in labial mucosa is different from that in the normal skin or oesophageal mucosa, although the labial mucosal epithelium, epidermis, and oesophageal epithelium are all stratified squamous epithelia. These differences in the cell-surface sugar residues are likely to be related to the possible functional differences in these tissues.
利用八种不同的凝集素（刀豆球蛋白A、扁豆凝集素、小麦胚凝集素、荆豆凝集素-1、蓖麻凝集素-1、大豆凝集素、麦芽凝集素和花生凝集素），通过光学显微镜和电子显微镜检查了正常人类唇黏膜中凝集素的结合模式，并与正常人类皮肤和食管黏膜中的模式进行了比较。光学显微镜下可见，刀豆球蛋白A、扁豆凝集素和小麦胚凝集素可使黏膜各层的细胞膜染色。蓖麻凝集素-1可使基底层和中间层细胞的质膜染色，而表层细胞几乎没有阳性染色。荆豆凝集素-1、大豆凝集素和花生凝集素在某些情况下可使中间层细胞弱阳性染色。未观察到麦芽凝集素的阳性染色。电子显微镜下，在质膜、内质网池、核膜和高尔基体中观察到了指示刀豆球蛋白A结合位点的反应产物。在质膜中观察到了扁豆凝集素、小麦胚凝集素和蓖麻凝集素-1的结合。这些结果表明，尽管唇黏膜上皮、表皮和食管上皮均为复层鳞状上皮，但唇黏膜中花生凝集素、大豆凝集素和蓖麻凝集素-1的结合模式与正常皮肤或食管黏膜中的不同。这些细胞表面糖残基的差异可能与这些组织中可能存在的功能差异有关。
