一种测定人乳中核黄素的简单方法。
A simple method for the determination of riboflavin in human milk.
作者信息
Rettenmaier R, Vuilleumier J P
出版信息
Int J Vitam Nutr Res. 1983;53(1):32-5.
PMID:6189796
Abstract
A fluorometric method for the determination of total riboflavin in human milk is described. Flavin-adenine dinucleotide (FAD) is hydrolyzed to riboflavin by enzyme treatment. Then the fluorescence of total riboflavin is measured in the aqueous phase at pH 4.5. The coefficient of variation was found to be 2.48% (n = 58). The procedure is simple, reproducible and allows the simultaneous analysis of a considerable number of samples. The recoveries are in the range of 95.8-100.8%.
摘要
描述了一种测定人乳中总核黄素的荧光法。通过酶处理将黄素腺嘌呤二核苷酸（FAD）水解为核黄素。然后在pH 4.5的水相中测量总核黄素的荧光。变异系数为2.48%（n = 58）。该方法简单、可重复，可同时分析大量样品。回收率在95.8 - 100.8%范围内。
相似文献
1
A simple method for the determination of riboflavin in human milk.一种测定人乳中核黄素的简单方法。
Int J Vitam Nutr Res. 1983;53(1):32-5.
2
Liquid chromatographic analysis of riboflavin vitamers in foods using fluorescence detection.采用荧光检测法对食品中核黄素维生素异构体进行液相色谱分析。
J Agric Food Chem. 2004 Apr 7;52(7):1789-94. doi: 10.1021/jf030756s.
3
Supercritical fluid extraction as an on-line clean-up technique for determination of riboflavin vitamins in food samples by capillary electrophoresis with fluorimetric detection.超临界流体萃取作为一种在线净化技术，用于通过荧光检测的毛细管电泳法测定食品样品中的核黄素类维生素。
Electrophoresis. 2008 Aug;29(15):3213-9. doi: 10.1002/elps.200800115.
4
[Spectrofluorimetric determination of FAD, FMN and free riboflavin in the blood].[血液中黄素腺嘌呤二核苷酸、黄素单核苷酸和游离核黄素的荧光分光光度法测定]
Cesk Farm. 1988 Apr;37(3):108-11.
5
Quantification of riboflavin, riboflavin 5'-phosphate and flavin adenine dinucleotide in plasma and urine by high-performance liquid chromatography.采用高效液相色谱法对血浆和尿液中的核黄素、核黄素5'-磷酸酯和黄素腺嘌呤二核苷酸进行定量分析。
J Chromatogr. 1987 Dec 25;423:105-13. doi: 10.1016/0378-4347(87)80332-8.
6
Quantification of riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in mammalian model cells by CE with LED-induced fluorescence detection.采用毛细管电泳-发光二极管诱导荧光检测法对哺乳动物模型细胞中的核黄素、黄素单核苷酸和黄素腺嘌呤二核苷酸进行定量分析。
Electrophoresis. 2015 Feb;36(4):518-25. doi: 10.1002/elps.201400451. Epub 2015 Jan 22.
7
Relation between riboflavin, flavin mononucleotide and flavin adenine dinucleotide concentrations in plasma and red cells in patients with critical illness.危重病患者血浆和红细胞中核黄素、黄素单核苷酸和黄素腺嘌呤二核苷酸浓度的关系。
Clin Chim Acta. 2010 Nov 11;411(21-22):1750-5. doi: 10.1016/j.cca.2010.07.024. Epub 2010 Jul 24.
8
Determination of riboflavin, flavin mononucletide and flavin adenine dinucleotide in biological tissues by capillary zone electrophoresis and laser-induced fluorescence detection.利用毛细管区带电泳和激光诱导荧光检测法测定生物组织中的核黄素、黄素单核苷酸和黄素腺嘌呤二核苷酸
Electrophoresis. 2001 Apr;22(6):1170-4. doi: 10.1002/1522-2683()22:6<1170::AID-ELPS1170>3.0.CO;2-1.
9
Optimizing separation conditions for riboflavin, flavin mononucleotide and flavin adenine dinucleotide in capillary zone electrophoresis with laser-induced fluorescence detection.利用激光诱导荧光检测优化毛细管区带电泳中核黄素、黄素单核苷酸和黄素腺嘌呤二核苷酸的分离条件。
J Chromatogr A. 2002 Aug 30;968(1-2):229-39. doi: 10.1016/s0021-9673(02)00953-6.
引用本文的文献
1
Micronutrients in Human Milk: Analytical Methods.人乳中的微量营养素：分析方法。
Adv Nutr. 2018 May 1;9(suppl_1):313S-331S. doi: 10.1093/advances/nmy017.
Zotero 插件