采用高压液相色谱法对人血清中25-羟基胆钙化醇进行定量分析。
Quantitation of 25-hydroxycholecalciferol in human serum by high-pressure liquid chromatography.
作者信息
Schaefer P C, Goldsmith R S
出版信息
J Lab Clin Med. 1978 Jan;91(1):104-8.
PMID:618982
Abstract
An HPLC method for assaying 25(OH)D3 in extracts of human serum is described. The method involves extraction of serum with methanol-dichloromethane and chromatography on a column of Sephadex LH-20 prior to HPLC with quantitation by UV absorbance. Comparison with a CBP assay for 25(OH)D3 showed no difference between the two methods.
摘要
描述了一种用于测定人血清提取物中25(OH)D3的高效液相色谱法。该方法包括用甲醇 - 二氯甲烷提取血清，并在高效液相色谱分析前在Sephadex LH - 20柱上进行色谱分离，通过紫外吸光度进行定量。与25(OH)D3的CBP测定法比较表明，两种方法之间没有差异。
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