Traba M L, Quesada M, Marín A, de la Piedra C, Babé M, Navarro F
Rev Esp Fisiol. 1984 Mar;40(1):69-76.
A precise specific method for measuring 25-hydroxycholecalciferol (25-OH CC) in 2 ml of human serum is described. It includes extraction with acetonitrile, separation with Sep-Pak C-18 cartridges, purification through HPLC and a further quantitation by means of a protein-binding assay. An exhaustive study of this protein-binding method has been performed. The sensitivity of the protein-binding assay is 20 pg/tube and the inter and intra-assay coefficients of variation are 9.6% and 8.7% respectively. The precision of the overall process has been assessed by calculating the inter and intra-assay coefficients of variation, 15.5 and 10.4% respectively. Mean serum value of 25-OH CC in normal subjects (18-50 years old, during spring) is 11.2 +/- 5.5 ng/ml.
本文描述了一种精确的特定方法，用于测量2ml人血清中的25-羟基胆钙化醇（25-OH CC）。该方法包括用乙腈萃取、用Sep-Pak C-18柱分离、通过高效液相色谱法纯化以及通过蛋白质结合测定法进一步定量。已对这种蛋白质结合方法进行了详尽的研究。蛋白质结合测定法的灵敏度为20pg/管，测定间和测定内变异系数分别为9.6%和8.7%。通过计算测定间和测定内变异系数分别为15.5%和10.4%，评估了整个过程的精密度。正常受试者（18-50岁，春季）的血清25-OH CC平均值为11.2±5.5ng/ml。
