Kinlaw C S, Robberson B L, Berget S M
J Biol Chem. 1983 Jun 10;258(11):7181-9.
Human small nuclear ribonucleoproteins (snRNPs) containing U1 and U2 snRNAs have been isolated from cultured cells by nonimmunological methods. The U1 snRNP population remained immunoprecipitable by systemic lupus erythematosis anti-RNP and anti-Sm antibodies throughout fractionation and contained polypeptides of molecular weights corresponding to those defined as U1 snRNP polypeptides by immunoprecipitation of crude extracts. The purified assemblies contained U1 RNA and nine snRNP polypeptides of molecular weights 67,000 (P67), 30,000 (P30), 23,000 (P23), 21,500 (P22), 17,500 (P18), 12,300 (P12), 10,200 (P10), 9,100 (P9), and 8,500 (P8). P67, P30, and P18 were unique to U1 snRNPs. The U2 snRNP population remained immunoprecipitable by the systemic lupus erythematosis anti-Sm antibody throughout fractionation. The purified U2 assemblies contained six polypeptides of molecular weights corresponding to those defined by immunoprecipitation to be common to U1 and U2 snRNPs including P23, P22, P12, P10, P9, and P8. In addition, U2 snRNPs contained a unique polypeptide of 27,000 Da.
已通过非免疫方法从培养细胞中分离出含有U1和U2小核核糖核蛋白(snRNPs)的人源小核核糖核蛋白。在整个分级分离过程中，U1 snRNP群体仍可被系统性红斑狼疮抗RNP和抗Sm抗体免疫沉淀，并且含有分子量与通过粗提物免疫沉淀定义为U1 snRNP多肽的分子量相对应的多肽。纯化的组装体包含U1 RNA和分子量分别为67,000 (P67)、30,000 (P30)、23,000 (P23)、21,500 (P22)、17,500 (P18)、12,300 (P12)、10,200 (P10)、9,100 (P9)和8,500 (P8)的九种snRNP多肽。P67、P30和P18是U1 snRNPs特有的。在整个分级分离过程中，U2 snRNP群体仍可被系统性红斑狼疮抗Sm抗体免疫沉淀。纯化的U2组装体包含六种多肽，其分子量与通过免疫沉淀定义为U1和U2 snRNPs共有的多肽分子量相对应，包括P23、P22、P12、P10、P9和P8。此外，U2 snRNPs含有一种独特的27,000 Da多肽。
