Hashimoto C, Steitz J A
Nucleic Acids Res. 1984 Apr 11;12(7):3283-93. doi: 10.1093/nar/12.7.3283.
U4 and U6 RNAs of mammalian cells possess extensive intermolecular sequence complementarity and hence have the potential to base pair. A U4/U6 RNA complex, detectable in nondenaturing polyacrylamide gels, is released when human small nuclear ribonucleoproteins (snRNPs) containing U1, U2, U4, U5, and U6 RNAs are dissociated with proteinase K in the presence of sodium dodecyl sulfate. The released RNA/RNA complex dissociates with increasing temperature, consistent with the existence of specific base-pairing between the two RNAs. Since U6 RNA is selectively released from intact snRNPs under the same conditions required to dissociate the U4/U6 RNA complex, the RNA-RNA interaction may be sufficient to maintain U4 and U6 RNAs in the same snRNP particle. The biological implications of these findings are discussed.
哺乳动物细胞的U4和U6 RNA具有广泛的分子间序列互补性，因此有碱基配对的潜力。在非变性聚丙烯酰胺凝胶中可检测到的U4/U6 RNA复合物，当含有U1、U2、U4、U5和U6 RNA的人小核核糖核蛋白（snRNP）在十二烷基硫酸钠存在下用蛋白酶K解离时会被释放出来。释放出的RNA/RNA复合物会随着温度升高而解离，这与两种RNA之间存在特异性碱基配对相一致。由于在解离U4/U6 RNA复合物所需的相同条件下，U6 RNA会从完整的snRNP中选择性释放出来，因此RNA-RNA相互作用可能足以将U4和U6 RNA维持在同一个snRNP颗粒中。本文讨论了这些发现的生物学意义。
