LaFond R E, Woodcock H, Woodcock C L, Kundahl E R, Lucas J J
J Cell Biol. 1983 Jun;96(6):1815-9. doi: 10.1083/jcb.96.6.1815.
When fused with mouse L-cell cytoplasts, chick erythrocyte nuclei enlarge, take up proteins from the host cytoplasm, and recommence RNA synthesis. We found that during this transition the erythrocyte nuclei gain an internal nuclear matrix, thus providing a novel approach to questions concerning the nature of the salt-resistant intranuclear skeleton. A new method for preparation and examination of the nuclear matrix in situ is also described.
当与小鼠L细胞胞质体融合时,鸡红细胞核会增大,从宿主细胞质中摄取蛋白质,并重新开始RNA合成。我们发现,在这个转变过程中,红细胞核获得了一个内部核基质,从而为有关耐盐性核内骨架性质的问题提供了一种新方法。本文还描述了一种原位制备和检测核基质的新方法。