Chen H Y, Sun J M, Hendzel M J, Rattner J B, Davie J R
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Manltoba, Winnipeg, Canada.
Biochem J. 1996 Nov 15;320 ( Pt 1)(Pt 1):257-65. doi: 10.1042/bj3200257.
The protein composition and structure of nuclear matrices isolated from adult chicken immature and mature erythrocytes were analysed. Visualization of nuclear matrices by electron microscopy showed that immature-erythrocyte nuclear matrices had internal structures, while most mature-erythrocyte nuclear matrices did not. Both mature- and immature-erythrocyte nuclear matrices were surrounded by a fibrous network of intermediate filaments. Two-dimensional gel electrophoretic analysis of proteins obtained from fractionated nuclear matrices led to the assignment of the proteins as components of the nuclear porelamina, internal matrix, or cytoskeleton. Common and different proteins belonging to one of the three groups were identified in nuclear matrices of immature and mature erythrocytes. Investigation of the partitioning of histone deacetylase activity, an enzyme associated with the internal matrix, among the erythroid nuclear matrix fractions provided evidence that mature- and immature-erythrocyte nuclear matrices have internal structures. However, the activity of histone deacetylase and level of internal matrix proteins from mature-erythrocyte nuclear matrices were less than those from immature-erythrocyte matrices. The low levels of nuclear RNA and internal matrix proteins may account for lack of visual evidence for an internal matrix in mature erythrocytes.
对从成年鸡未成熟和成熟红细胞中分离出的核基质的蛋白质组成和结构进行了分析。通过电子显微镜观察核基质发现，未成熟红细胞核基质具有内部结构，而大多数成熟红细胞核基质则没有。成熟和未成熟红细胞核基质均被中间丝的纤维网络所包围。对从分级分离的核基质中获得的蛋白质进行二维凝胶电泳分析，结果表明这些蛋白质可归为核孔层、内部基质或细胞骨架的组成成分。在未成熟和成熟红细胞的核基质中鉴定出了属于这三组之一的共同和不同蛋白质。对组蛋白脱乙酰酶活性（一种与内部基质相关的酶）在红细胞核基质组分中的分配情况进行研究，结果表明成熟和未成熟红细胞核基质均具有内部结构。然而，成熟红细胞核基质中组蛋白脱乙酰酶的活性和内部基质蛋白的水平低于未成熟红细胞基质。核RNA和内部基质蛋白水平较低可能是成熟红细胞中缺乏内部基质可视化证据的原因。
