一种用于检测疱疹病毒型特异性抗体的微量固相放射免疫测定法：标准化涉及的参数。
A micro solid-phase radioimmunoassay for detection of herpesvirus type-specific antibody: parameters involved in standardization.
作者信息
Matson D O, Adler-Storthz K, Adam E, Dreesman G R
出版信息
J Virol Methods. 1983 Feb;6(2):71-83. doi: 10.1016/0166-0934(83)90071-x.
A micro solid-phase radioimmunoassay (micro-SPRIA) was developed to demonstrate type-specific antibodies to herpes simplex virus types 1 and 2 (HSV1 and HSV2). Glycoproteins from the 123,000 dalton region of HSV1 (VP123) and the 119,000 dalton region of HSV2 (VP119) were isolated on preparative polyacrylamide gels for use as antigens in the micro-SPRIA. Human sera selected from clinical samples by virological history and appropriate microneutralization data were used to standardize the micro-SPRIA. Optimization of the assay required the use of siliconized microtiter wells for adsorption of antigen. Maximized results were highly dependent on the concentrations of antigen, primary antibody, and secondary antibody as well as the diluents used for these principal test reagents. Incorporation of HSV glycoproteins of each respective type with the optimal condition established in this study facilitates the direct detection of type-specific antibody in human sera.
开发了一种微量固相放射免疫测定法（micro-SPRIA）来检测针对单纯疱疹病毒1型和2型（HSV1和HSV2）的型特异性抗体。从HSV1的123,000道尔顿区域（VP123）和HSV2的119,000道尔顿区域（VP119）的糖蛋白在制备性聚丙烯酰胺凝胶上分离出来，用作micro-SPRIA中的抗原。根据病毒学病史和适当的微量中和数据从临床样本中选择的人血清用于标准化micro-SPRIA。该测定法的优化需要使用硅化微量滴定板来吸附抗原。最大化的结果高度依赖于抗原、一抗和二抗的浓度以及用于这些主要测试试剂的稀释剂。将每种类型的HSV糖蛋白与本研究中建立的最佳条件相结合，有助于直接检测人血清中的型特异性抗体。
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