Dreesman G R, Matson D O, Courtney R J, Adam E, Melnick J L
Intervirology. 1979;12(2):115-9. doi: 10.1159/000149077.
A micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The test is performed by coating wells of microtiter plates with purified HSV-specified glycoproteins, VP123 and VP119, derived from HSV-1- and HSV-2-infected cells, respectively. The coated wells are then incubated with diluted unadsorbed sera and subsequently with radioiodinated goat anti-human IgG reagent. Because these major viral envelope glycoproteins contain type-specific antigenic determinants, type-specific antibodies are preferentially detected in our assay.
已开发出一种微量固相放射免疫分析法，用于检测人血清中针对1型单纯疱疹病毒（HSV-1）和2型单纯疱疹病毒（HSV-2）的型特异性抗体。该检测方法是通过分别用从感染HSV-1和HSV-2的细胞中提取的纯化的HSV特异性糖蛋白VP123和VP119包被微量滴定板的孔来进行的。然后将包被好的孔与稀释的未吸附血清孵育，随后与放射性碘化山羊抗人IgG试剂孵育。由于这些主要的病毒包膜糖蛋白含有型特异性抗原决定簇，因此在我们的检测中可优先检测到型特异性抗体。
