Suchánková A, Hirsch I, Krĕmár M, Vonka V
J Infect Dis. 1984 Jun;149(6):964-72. doi: 10.1093/infdis/149.6.964.
Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) antibodies in humans and animals were determined by a micro-solid-phase radioimmunoassay with Helix pomatia lectin/Sepharose 4B-purified antigens from HSV-1- and HSV-2-infected cells. A low but constant heterotypic reactivity was found with HSV-1 antigen, whereas HSV-2 antigen was type specific, as evidenced by (1) its reactivity with sera from HSV-2- but not with those from HSV-1-immunized animals, (2) its reactivity with monoclonal antibody to glycoprotein C but not with that to other HSV-2 glycoproteins, and (3) the negative results obtained in serum samples from 57 children, of whom 49 possessed HSV-1 neutralizing antibody. Antibody to HSV-2 (anti-HSV-2) was detected in serum samples from 14 of 16 subjects treated for genital herpetic lesions. The prevalence of anti-HSV-2 in women did not differ in the 25-35- and 36-45-year-old age groups but did correlate with the number of sex partners, with only 10% of women reporting one sex partner and 33% of those reporting more than 10 sex partners having anti-HSV-2.
采用微固相放射免疫分析法，以从单纯疱疹病毒1型（HSV-1）和2型（HSV-2）感染细胞中经苹果蜗牛凝集素/琼脂糖4B纯化的抗原，测定人和动物体内的HSV-1和HSV-2抗体。发现HSV-1抗原存在低水平但恒定的异型反应性，而HSV-2抗原具有型特异性，这表现为：（1）其与HSV-2免疫动物的血清发生反应，而不与HSV-1免疫动物的血清反应；（2）其与糖蛋白C单克隆抗体发生反应，而不与其他HSV-2糖蛋白单克隆抗体反应；（3）在57名儿童的血清样本中得到阴性结果，其中49名儿童拥有HSV-1中和抗体。在16名接受生殖器疱疹病变治疗的受试者中，有14名的血清样本检测到HSV-2抗体（抗-HSV-2）。25至35岁和36至45岁年龄组女性的抗-HSV-2患病率无差异，但与性伴侣数量相关，报告有一名性伴侣的女性中只有10%有抗-HSV-2，而报告有超过10名性伴侣的女性中有33%有抗-HSV-2。
