Lindon J, Rosenberg R, Merrill E, Salzman E
J Lab Clin Med. 1978 Jan;91(1):47-59.
Platelet interaction with surfaces to which heparin had been covalently bonded was investigated with a chromatographic technique employing agarose gel beads heparinized via a cyanogen bromide reaction. Heparinization significantly increased platelet retention by the gel. Platelet retention was unchanged after pretreatment of the heparinized gel with albumin but increased after pretreatment with fibrinogen. Pretreatment with plasma or purified AT III decreased platelet retention. Reduction in platelet retention was correlated with the amount of AT III removed from plasma. Plasma with decreased levels of AT III was less effective in surface passivation. Pretreatment of heparinized gel with PF4 or protamine sulfate did not decrease platelet retention, but subsequent exposure to plasma did. The results suggest that a surface with covalently bonded heparin is reactive toward platelets but can be passivated by formation of a heparin/AT III complex.
采用通过溴化氰反应肝素化的琼脂糖凝胶珠的色谱技术，研究了血小板与共价结合肝素的表面的相互作用。肝素化显著增加了凝胶对血小板的保留。用白蛋白预处理肝素化凝胶后，血小板保留率不变，但用纤维蛋白原预处理后增加。用血浆或纯化的抗凝血酶III预处理可降低血小板保留率。血小板保留率的降低与从血浆中去除的抗凝血酶III的量相关。抗凝血酶III水平降低的血浆在表面钝化方面效果较差。用PF4或硫酸鱼精蛋白预处理肝素化凝胶不会降低血小板保留率，但随后暴露于血浆中则会降低。结果表明，共价结合肝素的表面对血小板具有反应性，但可通过形成肝素/抗凝血酶III复合物而被钝化。
