Dennert G, Podack E R
J Exp Med. 1983 May 1;157(5):1483-95. doi: 10.1084/jem.157.5.1483.
Cloned T killer cells derived from one-way mixed lymphocyte reactions were characterized with regard to their Lyt phenotype and specificity. Two clones of Lyt-1-2+ phenotype that recognized H-2Dd were selected and examined for their cytolytic function by negative staining and thin section electron microscopy. When incubated with the H-2d target S194, they assemble two types of tubular complexes, polyperforin 1 and 2. Both structures appear to arise by polymerization of precursors that may originate in dense granules and/or Golgi of T killer cells. Polyperforins appear to be associated with vesicles that are released during the lytic reaction and transferred to target membranes as shown by immunoelectron microscopy. Since there is a close correlation between target lysis and the appearance of polyperforins on target membranes, it is suggested that polyperforins take part in the cell-mediated killing reaction. Although polyperforins are different in size and several molecular properties from complement, there are striking similarities between these circular complexes and polyperforin (C9). It is therefore possible that they belong to a closely related family of cytolytic effector molecules.
对源自单向混合淋巴细胞反应的克隆T杀伤细胞进行了Lyt表型和特异性方面的鉴定。选择了两个识别H-2Dd的Lyt-1-2+表型克隆，并通过负染色和超薄切片电子显微镜检查它们的细胞溶解功能。当与H-2d靶细胞S194孵育时，它们组装出两种类型的管状复合物，即多穿孔素1和2。这两种结构似乎都是由可能起源于T杀伤细胞致密颗粒和/或高尔基体的前体聚合而成。如免疫电子显微镜所示，多穿孔素似乎与在裂解反应期间释放并转移到靶膜上的囊泡相关。由于靶细胞裂解与靶膜上多穿孔素的出现密切相关，因此提示多穿孔素参与细胞介导的杀伤反应。尽管多穿孔素在大小和一些分子特性上与补体不同，但这些环状复合物与多穿孔素（C9）之间存在显著相似性。因此，它们有可能属于一个密切相关的细胞溶解效应分子家族。
