Koo G C, Jacobson J B, Hammerling G J, Hammerling U
J Immunol. 1980 Sep;125(3):1003-6.
We have determined the antigenic profile of natural killer (NK) cells from C57BL/6 mice that have lytic activity to RLmale1 tumor cells, in a 5-hr 51Cr release assay. To perform the antigenic typing more effectively, we enriched the NK populations by using discontinous BSA density gradient and established a correlation between NK activity and NK-1 antigen in each layer. As NK activity and Nk-1 antigen are both criteria for measuring NK cells, we determined the antigenic profile of NK cells by monitoring both the change in NK activity and NK-1 cells, after elimination of cells with various antisera to lymphocytic alloantigens. We have found that all NK cells expressed Nk-1, Qa-4, and Qa-5 antigens; about 50% of NK cells expressed Thy-1 and Qa-2 antigens, and 25% of NK cells also expressed Lyt-1 antigen. NK cells did not express significant amounts of Qa-1, Qa-3, Lyt-2, and Lyb-2 antigens. Therefore, spontaneous NK cells are heterogeneous with regard to their surface antigens, some of which are T cell antigens. However, the present antigenic profile does not allow us to classify NK cells in the conventional T cells lineage.
我们在一项5小时的51Cr释放试验中，确定了对RLmale1肿瘤细胞具有裂解活性的C57BL/6小鼠自然杀伤（NK）细胞的抗原谱。为了更有效地进行抗原分型，我们使用不连续牛血清白蛋白密度梯度富集NK细胞群体，并在每一层中建立了NK活性与NK-1抗原之间的相关性。由于NK活性和Nk-1抗原都是衡量NK细胞的标准，我们通过用各种淋巴细胞同种异体抗原抗血清清除细胞后，监测NK活性和NK-1细胞的变化，来确定NK细胞的抗原谱。我们发现，所有NK细胞均表达Nk-1、Qa-4和Qa-5抗原；约50%的NK细胞表达Thy-1和Qa-2抗原，25%的NK细胞还表达Lyt-1抗原。NK细胞不表达大量的Qa-1、Qa-3、Lyt-2和Lyb-2抗原。因此，自发NK细胞在其表面抗原方面是异质性的，其中一些是T细胞抗原。然而，目前的抗原谱不允许我们将NK细胞归类于传统的T细胞谱系。
