Busslinger M, deBoer E, Wright S, Grosveld F G, Flavell R A
Nucleic Acids Res. 1983 Jun 11;11(11):3559-69. doi: 10.1093/nar/11.11.3559.
MspI essentially fails to cut the sequence GGCmCGG at enzyme concentrations which give total digestion of CCGG, CmCGG and GGCCGG sites. This result explains why certain sites in mammalian DNA are resistant to both MspI and HpaII and shows that this results from an idiosynchracy of MspI rather than a novel form of DNA methylation at this site in mammalian cells.
在能够完全酶切CCGG、CmCGG和GGCCGG位点的酶浓度下，MspI基本无法切割GGCmCGG序列。这一结果解释了为什么哺乳动物DNA中的某些位点对MspI和HpaII均具有抗性，表明这是由MspI的特异性造成的，而非哺乳动物细胞中该位点存在新形式的DNA甲基化。
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