Tang F, Costa E, Schwartz J P
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3841-4. doi: 10.1073/pnas.80.12.3841.
Proenkephalin mRNA has been detected in striatum, hypothalamus, cortex, cerebellum, hippocampus, midbrain, and brain stem of rat by RNA ("Northern") blot analysis using a 918-base-pair DNA hybridization probe complementary to proenkephalin mRNA [Comb, M., Seeburg, P. H., Adelman, J., Eiden, L. & Herbert, E. (1982) Nature (London) 295, 663-666]. The size of the mRNA species in all brain regions is approximately 1,400 bases, and it was found to be comparable with that of bovine adrenal medulla and human pheochromocytoma. The data were quantified by densitometric scanning of the autoradiograms: the area under the peak is proportional to the amount of standard proenkephalin mRNA from bovine adrenal medulla. The relative content of proenkephalin mRNA in the various brain regions correlates generally with the content of [Met5]enkephalin-like immunoactivity of these regions. Rats receiving daily intraperitoneal injection of haloperidol (1 or 2 mg/kg) show a fourfold increase of proenkephalin mRNA content in striatum but not other brain regions. In agreement with previous reports, [Met5]enkephalin-like immunoactivity increased twofold in striatum.
利用与前脑啡肽原mRNA互补的918个碱基对的DNA杂交探针，通过RNA（“Northern”）印迹分析在大鼠的纹状体、下丘脑、皮层、小脑、海马、中脑和脑干中检测到了前脑啡肽原mRNA[Comb, M., 西伯尔格, P. H., 阿德尔曼, J., 艾登, L. & 赫伯特, E. (1982) 《自然》（伦敦）295, 663 - 666]。所有脑区中mRNA种类的大小约为1400个碱基，并且发现其与牛肾上腺髓质和人嗜铬细胞瘤的mRNA大小相当。通过对放射自显影片进行光密度扫描对数据进行定量：峰下面积与来自牛肾上腺髓质的标准前脑啡肽原mRNA的量成正比。前脑啡肽原mRNA在各个脑区中的相对含量通常与这些区域中[Met5]脑啡肽样免疫活性的含量相关。每日腹腔注射氟哌啶醇（1或2毫克/千克）的大鼠，其纹状体中的前脑啡肽原mRNA含量增加了四倍，但其他脑区未增加。与先前的报道一致，纹状体中[Met5]脑啡肽样免疫活性增加了两倍。
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