Murai T, Andrews J W
J Nutr. 1978 Jan;108(1):176-80. doi: 10.1093/jn/108.1.176.
In separate 20 and 12 week feeding studies, channel catfish fingerlings were fed semipurified diets containing five levels (0, 2.5, 5.0, 10.0 and 15.0 mg/kg) and six levels (0, 0.5, 1.0, 2.5, 5.0 and 10.0 mg/kg) of supplemented thiamin hydrochloride respectively. The dietary thiamin level required to provide maximal growth and prevent deficiency symptoms in channel catfish fingerlings was found to be approximately 1 mg/kg of diet. Fish fed unsupplemented diets (thiamin content of less 1 mg/kg diet) demonstrated anorexia, extremely poor growth, dark coloration of the skin and increased mortality rates. Neurological symptoms were not observed. Histological examinations of the heart, hepatopancreas, kidney, lateral muscle, gastrointestinal tract and gills of deficient fish revealed no abnormalities.
在两项分别为期20周和12周的饲养研究中，分别给斑点叉尾鮰鱼苗投喂含有五个水平（0、2.5、5.0、10.0和15.0毫克/千克）和六个水平（0、0.5、1.0、2.5、5.0和10.0毫克/千克）添加盐酸硫胺素的半纯化日粮。结果发现，为使斑点叉尾鮰鱼苗实现最大生长并预防缺乏症状所需的日粮硫胺素水平约为1毫克/千克日粮。投喂未添加硫胺素日粮（硫胺素含量低于1毫克/千克日粮）的鱼出现厌食、生长极差、皮肤颜色变深以及死亡率增加的情况。未观察到神经症状。对缺乏硫胺素的鱼的心脏、肝胰腺、肾脏、侧肌、胃肠道和鳃进行组织学检查，未发现异常。
