Shatzman A R, Henkin R I
Biochem Med. 1983 Apr;29(2):182-93. doi: 10.1016/0006-2944(83)90039-x.
The major proteins in human parotid saliva, isolated in Fractions II-V following chromatography on Sephacryl S-200, DEAE-Sephadex A-50, or CM cellulose, contain 6 moles of phosphate per mole of protein, the phosphate probably bound to the protein via an ester linkage. This phosphate represents greater than 90% of the protein-bound phosphate in human parotid saliva. Neither purified gustin nor amylase, the two other major proteins in human parotid saliva, contain phosphate.
在Sephacryl S - 200、DEAE - Sephadex A - 50或CM纤维素上进行色谱分离后,在组分II - V中分离出的人腮腺唾液中的主要蛋白质,每摩尔蛋白质含有6摩尔磷酸盐,磷酸盐可能通过酯键与蛋白质结合。这种磷酸盐占人腮腺唾液中蛋白质结合磷酸盐的90%以上。人腮腺唾液中的另外两种主要蛋白质,纯化的味觉素和淀粉酶,都不含磷酸盐。