Thiébaud C H
Dev Biol. 1983 Jul;98(1):245-9. doi: 10.1016/0012-1606(83)90353-6.
A new reliable and durable method for marking cells in Xenopus is described. It is based on the differential staining of the nuclei of different Xenopus species, e.g., X. laevis and X. borealis, with the fluorescent dye quinacrine. This method permits us to recognize with certainty each cell in mitosis and interphase of X. borealis origin in any tissue combination with most of the other Xenopus species tested so far. This holds for all stages of development following grafting experiments, including adult tissues. The method is applicable in smears and squash preparations as well as in microtome sections. The method is particularly useful for marking migrating cells which are difficult to track, for instance, in embryos and in the circulatory system.
本文描述了一种用于标记非洲爪蟾细胞的新型可靠且持久的方法。该方法基于用荧光染料喹吖因对不同非洲爪蟾物种（如光滑爪蟾和北美爪蟾）的细胞核进行差异染色。此方法使我们能够确定在与迄今测试的大多数其他非洲爪蟾物种的任何组织组合中，源自北美爪蟾处于有丝分裂和间期的每个细胞。这适用于移植实验后的所有发育阶段，包括成体组织。该方法适用于涂片、压片标本以及切片。该方法对于标记难以追踪的迁移细胞特别有用，例如在胚胎和循环系统中的细胞。
