Asfari M, Thiébaud C H
Département de Biologie Animale, Université de Genève, Switzerland.
Cancer Res. 1988 Feb 15;48(4):954-7.
The fate of the putative transplantable Xenopus lymphosarcoma (M. Balls, Cancer Res., 22: 1142-1154, 1962) was studied under three experimental conditions: (a) xenotransplantation, i.e., transplantation of live "tumor" tissue between adults of Xenopus borealis and X. laevis; (b) inoculation of live "tumor" cells from X. borealis into the blastocoele of X. laevis embryos; and (c) transplantation of "tumor" tissue into recipient adults immunologically unresponsive to the donor tissue antigens. This condition is fulfilled by using X. laevis-X. gilli (LG) hybrids [H.R. Kobel and L. Du Pasquier. In: J.B. Solomon and J.D. Horton (eds.), Developmental Immunology, pp. 229-306. The Netherlands: Elsevier/North-Holland Biomedical Press, 1977] as donors, and triploid X. laevis-X. gilli and X. borealis (LGB) hybrids [C.H. Thiébaud, Dev. Biol., 98: 245-249, 1983] as recipients. In all transplantation experiments, donor and recipient cells could be unambiguously distinguished upon quinacrine staining that yields typical nuclear patterns, for instance bright patchiness in X. borealis, visible also in LGB cells. The results of xenotransplantation between X. borealis and X. laevis indicated that all developing "tumors" were composed of the recipient cell phenotype. The inoculation of live "tumor" cells from X. borealis "tumor" into the blastocoele of X. laevis embryos resulted in "tumor" formation in the recipient tadpoles and in metamorphosed animals. The cell constituting these "tumors" all were of recipient, X. laevis cell phenotype. Finally, "tumor" tissues from LG clones transplanted into LGB hosts were replaced by "tumors" formed of cells with recipient, LGB phenotype. These experiments indicate that this Xenopus tumor-like growth is a transmissible and not a transplantable disorder.
在三种实验条件下研究了假定可移植的非洲爪蟾淋巴肉瘤（M. 鲍尔斯，《癌症研究》，22: 1142 - 1154，1962）的命运：（a）异种移植，即北方爪蟾和非洲爪蟾成体之间活体“肿瘤”组织的移植；（b）将北方爪蟾的活体“肿瘤”细胞接种到非洲爪蟾胚胎的囊胚腔中；以及（c）将“肿瘤”组织移植到对供体组织抗原免疫无反应的受体成体中。通过使用非洲爪蟾 - 吉利爪蟾（LG）杂种[H.R. 科贝尔和L. 迪帕斯奎尔。载于：J.B. 所罗门和J.D. 霍顿（编），《发育免疫学》，第229 - 306页。荷兰：爱思唯尔/北荷兰生物医学出版社，1977]作为供体，以及三倍体非洲爪蟾 - 吉利爪蟾和北方爪蟾（LGB）杂种[C.H. 蒂博，《发育生物学》，98: 245 - 249，1983]作为受体来满足这一条件。在所有移植实验中，通过喹吖因染色可明确区分供体细胞和受体细胞，喹吖因染色会产生典型的核模式，例如北方爪蟾中的明亮斑点，在LGB细胞中也可见。北方爪蟾和非洲爪蟾之间异种移植的结果表明，所有发育中的“肿瘤”均由受体细胞表型组成。将北方爪蟾“肿瘤”的活体“肿瘤”细胞接种到非洲爪蟾胚胎的囊胚腔中，导致受体蝌蚪和变态后的动物中形成“肿瘤”。构成这些“肿瘤”的细胞均为受体非洲爪蟾细胞表型。最后，从LG克隆移植到LGB宿主中的“肿瘤”组织被由具有受体LGB表型的细胞形成的“肿瘤”所取代。这些实验表明，这种非洲爪蟾类肿瘤样生长是一种可传播的疾病，而非可移植性疾病。
