Mackin W M, Mayer E P, Dray S, Reiter H
Immunology. 1983 Jul;49(3):519-28.
Approximately half of the rosettes formed by rabbit Ig+ lymphocytes (B cells) and anti-coated erythrocytes or glutaraldehyde-fixed bacteria are dissociated upon the addition of rabbit serum. Rabbit serum was fractionated and the rosette-dissociating activity was found in purified preparations of rabbit alpha 1- and alpha 2-macroglobulins. Studies designed to elucidate the mechanism of rosette dissociation suggested that the alpha-macroglobulins dissociated rosettes by causing the release or proteolytic cleavage of the membrane proteins complexed with the anti-Ig-coated particles. These data suggest that the alpha-macroglobulins may have a role in the interaction of B lymphocytes with particulate antigens.
由兔Ig⁺淋巴细胞(B细胞)与抗包被红细胞或戊二醛固定细菌形成的玫瑰花结,在加入兔血清后约有一半会解离。对兔血清进行了分级分离,发现兔α1和α2巨球蛋白的纯化制剂中具有玫瑰花结解离活性。旨在阐明玫瑰花结解离机制的研究表明,α巨球蛋白通过导致与抗Ig包被颗粒结合的膜蛋白释放或蛋白水解裂解来解离玫瑰花结。这些数据表明,α巨球蛋白可能在B淋巴细胞与颗粒性抗原的相互作用中发挥作用。