Calder E A, Urbaniak S J, Irvine W J, James K
Clin Exp Immunol. 1975 Oct;22(1):112-20.
The effect of antisera to human alpha2-macroglobulin (alpha2M) on the ability of human peripheral blood lymphoid cells to lyse antibody coated target cells (exert a K-cell effect) and form T(E) and B(EAC) rosettes has been investigated. Pretreatment of lymphoid cells with these antisera inhibited their K-cell activity but had no effect on their capacity to form T or B rosettes. The inhibitory activity of the anti-alpha2M sera was always reduced following absorption on a CnBr column to which human alpha2M had been coupled. The absorbed antibody, recovered by acid elution reacted strongly with human alpha2M in gel diffusion precipitin analysis and caused a highly significant inhibition of K-cell cytotoxicity. It was further shown that the inhibitory activity of the anti-alpha2M was localized exclusively in the IgG fraction and was greatly reduced following pepsin digestion.
已研究了抗人α2-巨球蛋白（α2M）抗血清对人外周血淋巴细胞溶解抗体包被靶细胞（发挥K细胞效应）以及形成T（E）和B（EAC）玫瑰花结能力的影响。用这些抗血清预处理淋巴细胞会抑制其K细胞活性，但对其形成T或B玫瑰花结的能力没有影响。抗α2M血清的抑制活性在与人α2M偶联的溴化氰柱上吸附后总是会降低。通过酸洗脱回收的吸附抗体在凝胶扩散沉淀分析中与人α2M强烈反应，并对K细胞细胞毒性产生高度显著的抑制作用。进一步表明，抗α2M的抑制活性仅定位于IgG组分，并且在胃蛋白酶消化后大大降低。
