Chechik B E, Sengupta S
J Immunol Methods. 1981;45(2):165-76. doi: 10.1016/0022-1759(81)90210-6.
A commercial preparation of calf adenosine deaminase (calf ADA) was further purified by affinity chromatography and used for immunization of rabbits. The resulting anti-calf-ADA sera reacted by immunodiffusion with both calf and human ADA, and precipitated about 90% of radiolabeled enzyme isolated from human thymus tissue. Moreover, ADA activity was detected in the pellets formed by immunoprecipitation of unlabeled human enzyme by anti-calf-ADA sera. These antisera were successfully used for the immunomorphologic localization of ADA in human thymus tissue and in lymphoid cell preparations. The anti-calf ADA sera could also be used for the immunofluorescent detection of enzyme in rat and mouse thymocytes. The utilization of anti-calf-ADA serum for immunochemical and immunomorphologic detection of enzyme provides a valuable and sensitive reagent for the identification of ADA-positive cells in humans and several other species.
一种市售的小牛腺苷脱氨酶（小牛ADA）制剂通过亲和层析进一步纯化，并用于免疫兔子。所得的抗小牛ADA血清通过免疫扩散与小牛和人ADA发生反应，并沉淀出约90%从人胸腺组织中分离出的放射性标记酶。此外，在抗小牛ADA血清对未标记人酶进行免疫沉淀形成的沉淀中检测到ADA活性。这些抗血清成功用于人胸腺组织和淋巴细胞制剂中ADA的免疫形态定位。抗小牛ADA血清也可用于大鼠和小鼠胸腺细胞中酶的免疫荧光检测。利用抗小牛ADA血清进行酶的免疫化学和免疫形态检测，为鉴定人和其他几种物种中的ADA阳性细胞提供了一种有价值且灵敏的试剂。
