Auriault C, Capron M, Cesari I M, Capron A
J Immunol. 1983 Jul;131(1):464-70.
Schistosomulum-released products (SRP) have been shown to enhance both expression of rat and human eosinophil Fc receptors and IgG-dependent cytotoxicity. The present work provides additional evidence of the secretion of eosinophil-enhancing factors by schistosomula and other developmental stages of schistosomes, including adult worms. The heat lability, as well as the strong inhibition of the stimulating activity of SRP by the protease inhibitor Trasylol, suggest that thermolabile proteases secreted by the parasite are involved in this mechanism. The purification of the schistosome proteases by preparative isoelectric focusing and gel filtration demonstrated that neutral proteases able to hydrolyze the collagenase substrates Azocoll and Z-Gly-Pro-Leu-Gly-Pro are able to significantly enhance eosinophil effector functions. Purified Clostridium histolyticum collagenase was also able to mimic the enhancing effect of schistosome proteases, suggesting involvement of a collagenase-like activity of the enzymes in the eosinophil stimulation.
已证明血吸虫童虫释放产物（SRP）可增强大鼠和人类嗜酸性粒细胞Fc受体的表达以及IgG依赖性细胞毒性。目前的研究工作提供了额外证据，表明血吸虫童虫以及血吸虫的其他发育阶段（包括成虫）会分泌嗜酸性粒细胞增强因子。热不稳定性以及蛋白酶抑制剂抑肽酶对SRP刺激活性的强烈抑制作用表明，寄生虫分泌的热不稳定蛋白酶参与了这一机制。通过制备性等电聚焦和凝胶过滤对血吸虫蛋白酶进行纯化，结果表明能够水解胶原酶底物偶氮胶原和Z-甘氨酰-脯氨酰-亮氨酰-甘氨酰-脯氨酸的中性蛋白酶能够显著增强嗜酸性粒细胞的效应功能。纯化的溶组织梭菌胶原酶也能够模拟血吸虫蛋白酶的增强作用，这表明这些酶的类胶原酶活性参与了对嗜酸性粒细胞的刺激。
