Wainer I W, Stambaugh J E
J Pharm Sci. 1978 Jan;67(1):116-8. doi: 10.1002/jps.2600670131.
A GLC procedure for the determination of meperidinic and normeperidinic acids in human urine is described. After the extraction of any interfering meperidine or normeperidine, the urine samples are dried and the acids are reesterified using ethanol-sulfuric acid. The resulting meperidine and normeperidine are then extracted and quantified. With this method, the urinary excretion of these metabolites was followed in five subjects who received a single meperidine dose of 36 mg/m2 im. This method represents an improvement over the previously described methods for meperidinic and normeperidinic acids and can be applied to clinical situations.
本文描述了一种用于测定人尿液中哌替啶酸和去甲哌替啶酸的气相色谱法。在提取任何干扰性的哌替啶或去甲哌替啶后，将尿液样本干燥，然后用乙醇 - 硫酸将酸重新酯化。随后提取并定量生成的哌替啶和去甲哌替啶。采用该方法，对五名接受36 mg/m² 哌替啶单次肌肉注射的受试者的这些代谢物的尿排泄情况进行了跟踪。该方法相较于先前描述的测定哌替啶酸和去甲哌替啶酸的方法有所改进，可应用于临床情况。
