[采用气相色谱/氢火焰离子化检测器和气相色谱/质谱联用技术分析一名成瘾者尿液中的哌替啶及其代谢产物]

[Analysis of meperidine and its metabolites in urine of an addict by GC/FID and GC/MS].

作者信息

Hu X Y, Liu F, Luo Y

机构信息

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing.

出版信息

Yao Xue Xue Bao. 1994;29(2):116-21.

PMID:8042511

Abstract

Analytical methods of GC/FID and GC/MS for identification and determination of meperidine, an analgesic drug, and its metabolites were established. The determination of meperidine spiked in urine was shown to have a good linear relationship in the range of 0.1-8.0 micrograms/ml urine (Y = 0.4995X + 0.1201, r = 0.9996). The detection limit was 20 ng/ml urine. With the developed methods, meperidine and its metabolites in urine collected from a meperidine addict were identified and determined. Meperidine, normeperidine, meperidinic acid in free and conjugate forms, normeperidine acid in free and conjugate forms, and acetyl-normeperidine were detected. Among these metabolites, acetyl-normeperidine was reported for the first time.

摘要

建立了气相色谱/氢火焰离子化检测器（GC/FID）和气相色谱/质谱联用（GC/MS）用于鉴定和测定镇痛药哌替啶及其代谢物的分析方法。结果表明，尿液中加标的哌替啶在0.1 - 8.0微克/毫升尿液范围内具有良好的线性关系（Y = 0.4995X + 0.1201，r = 0.9996）。检测限为20纳克/毫升尿液。采用所建立的方法，对一名哌替啶成瘾者尿液中的哌替啶及其代谢物进行了鉴定和测定。检测到了哌替啶、去甲哌替啶、游离及结合形式的哌替啶酸、游离及结合形式的去甲哌替啶酸以及乙酰去甲哌替啶。其中，乙酰去甲哌替啶为首次报道。