[Detection of pregnancy-specific beta 1-glycoprotein (SP1), of proteins of the "pregnancy zone" (PZ), of pregnancy-associated plasma protein A (PAPP-A) with 24-hour radial immunodiffusion. Detection of pregnancy-specific beta 1-glycoprotein (SP1) with turbidimetry. Current significance of the proteins].

The estimations of pregnancy specific beta 1-glycoprotein (SP1), pregnancy associated alpha 2-glycoprotein (PZ) and pregnancy associated plasma protein A (PAPP-A) in serum samples are useful for supervision of pregnancy and diagnosis of gestational diseases. In clinical practice simple inexpensive and short time methods are preferred. Two simple short time methods were described. The 24 hour radial immuno-diffusion (RID) was compared with the usual 48 hour RID in 30 serum samples of normal pregnant women. The SP1-values ascertained with the 24 hour RID and the 48 hour RID correlated with a high coefficient (r = 0.99). In ranges of high SP1-concentrations the correlation was decreased. The correlation coefficients for PZ and PAPP-A amounted to 0.89 and 0.925. The estimation of proteins with turbidimetry is a real short time method. This method was performed in 19 serum samples of pregnant women and the results were compared with the 24 hour RID. The correlation coefficient amounted to 0.81. Between the results of 24 hour RID for SP1 and PAPP-A there was no correlation.