Murata A, Ogawa M, Fujimoto K, Kitahara T, Matsuda Y, Kosaki G
Enzyme. 1983;30(1):29-37. doi: 10.1159/000469542.
A radioimmunoassay for determination of human pancreatic elastase 1 was developed and the interaction of pancreatic elastase 1 with serum protease inhibitors (alpha 2-macroglobulin and alpha 1-antitrypsin) was investigated. Gel filtration studies showed that immunoreactive elastase 1 was present in serum and intact plasma as a complex with alpha 1-antitrypsin. Proelastase 1 in human pancreatic juice did not bind to protease inhibitors. But, after incubation with human serum or after activation with bovine trypsin, it was present as a complex with alpha 1-antitrypsin. The pancreatic elastase 1 content of the serum, measured by radioimmunoassay, was found to be influenced by the contents of both serum alpha 2-macroglobulin and alpha 1-antitrypsin. A significant inverse correlation was observed between the serum immunoreactive elastase 1 and alpha 2-macroglobulin contents in various hepatic diseases. The concentration of serum immunoreactive pancreatic elastase 1 was 1.79 +/- 0.62 ng/ml (mean +/- SD) in 28 normal controls. Its concentration was significantly increased in patients with acute pancreatitis, and although its concentration varied widely in patients with pancreatic cancer, its average concentration in these patients was significantly elevated.
建立了一种用于测定人胰腺弹性蛋白酶1的放射免疫分析方法，并研究了胰腺弹性蛋白酶1与血清蛋白酶抑制剂（α2-巨球蛋白和α1-抗胰蛋白酶）的相互作用。凝胶过滤研究表明，免疫反应性弹性蛋白酶1在血清和完整血浆中以与α1-抗胰蛋白酶的复合物形式存在。人胰液中的弹性蛋白酶原1不与蛋白酶抑制剂结合。但是，与人血清孵育后或用牛胰蛋白酶激活后，它以与α1-抗胰蛋白酶的复合物形式存在。通过放射免疫分析测定的血清中胰腺弹性蛋白酶1的含量受血清α2-巨球蛋白和α1-抗胰蛋白酶含量的影响。在各种肝病中，观察到血清免疫反应性弹性蛋白酶1与α2-巨球蛋白含量之间存在显著的负相关。28名正常对照者血清免疫反应性胰腺弹性蛋白酶1的浓度为1.79±0.62 ng/ml（平均值±标准差）。急性胰腺炎患者其浓度显著升高，胰腺癌患者其浓度虽变化很大，但其平均浓度在这些患者中也显著升高。
