Zhou H, Sziegoleit A, Fischer H P
Pathologisches Institut, Universität Bonn, Germany.
Histochem Cell Biol. 1995 Feb;103(2):103-9. doi: 10.1007/BF01454006.
By light and electron microscopic immunocytochemistry the distribution is described of human pancreatic elastase 1 (E1) during ontogenesis, in adults, in cases of acute and chronic pancreatitis, acute pancreatic ischaemia as well as pancreatic tumours. E1-positive cells were first detected in ductal sprouts in the 14th gestational week. Complete acini expressing E1 could be found from the 17th to the 20th week of gestation onwards. Scattered distinct E1-positive epithelia could be found in the ducts of fetal and adult pancreas. By immunoelectron microscopy, E1 was localized in rough endoplasmic reticulum, condensing vacuoles, zymogen granules of acinar epithelia and in acinar lumina. E1 appeared to be distributed homogeneously in zymogen granules. As specific markers of acinar cells, both monoclonal antibodies under study identified heterotopic pancreatic acini in peribiliar glands of the liver and also helped to visualize different damage patterns in pancreatitis. The acinar epithelia surrounding acute lipolytic necroses initially reacted more intensely with the E1-antibodies than undamaged pancreatic tissue. In acute ischaemia, acinar cells which are dissociated from intercalated ducts lost their immunocytochemical reactivity for E1. Pancreatic parenchyma involved in advanced acute pancreatitis as well as in chronic inflammation was detected only weakly by both E1-antibodies. However, atrophic lobules in post-inflammatory scars were stained more intensely by the E1-antibodies than normal parenchyma. Pancreatic tumours (adenomas, adenocarcinomas, solid-cystic tumours and islet cell tumours) were not labelled by these antibodies.
通过光镜和电镜免疫细胞化学方法，描述了人胰腺弹性蛋白酶1（E1）在个体发育过程中、成人、急性和慢性胰腺炎、急性胰腺缺血以及胰腺肿瘤中的分布情况。在妊娠第14周时，首次在导管芽中检测到E1阳性细胞。从妊娠第17周到20周起，可发现完整表达E1的腺泡。在胎儿和成人胰腺的导管中可发现散在的明显E1阳性上皮细胞。通过免疫电子显微镜观察，E1定位于粗面内质网、浓缩泡、腺泡上皮细胞的酶原颗粒以及腺泡腔内。E1似乎在酶原颗粒中均匀分布。作为腺泡细胞的特异性标志物，所研究的两种单克隆抗体均鉴定出肝脏胆管周围腺体中的异位胰腺腺泡，并且有助于观察胰腺炎中的不同损伤模式。急性脂肪溶解性坏死周围的腺泡上皮最初与E1抗体的反应比未受损的胰腺组织更强烈。在急性缺血时，从闰管分离的腺泡细胞失去了对E1的免疫细胞化学反应性。参与晚期急性胰腺炎以及慢性炎症的胰腺实质，用两种E1抗体检测时均显示弱阳性。然而，炎症后瘢痕中的萎缩小叶比正常实质被E1抗体染色更强烈。胰腺肿瘤（腺瘤、腺癌、实性囊性肿瘤和胰岛细胞瘤）未被这些抗体标记。
